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. 2022 Apr 13;154(5):e202112865. doi: 10.1085/jgp.202112865

Video 2.

Confocal live-cell imaging of Jurkat cells transiently expressing GFP-tagged NFAT. In the resting state, GFP fluorescence is predominantly visible in the cytosol. 8 min after stimulation with 5 Gy x rays, NFAT-GFP fluorescence alters its location and progressively moves over a period of ∼30 min completely into the nucleus. Time in h:min; images taken with a frame rate of one/5 s.