Extended Data Fig. 2 |. ENZ-resistant lineage plastic tumours exhibit a distinct AR cistrome.

(a) Principal component analysis (PCA) reveals distinct AR binding patterns across prostate states. Each dot represents the genome-wide AR cistrome in an individual clinical specimen (6 normal prostate epithelial, 18 primary prostate cancer tumours, 15 PDX tumours derived from patient mCRPC; GEO:GSE130408) or indicated cell line. (b) Gene ontology (GO) pathways enriched surrounding AR binding sites in ENZ-resistant AR-driven (49F^ENZR) and lineage plastic (42D^ENZR) cells. The closest 2000 peaks in proximity to a transcriptional start site were used for pathway analysis. Statistical significance was determined using a hypergeometric test. Representative AR ChIP-seq tracks surrounding the KLK3/PSA locus are shown. (c) Heatmap indicating AR ChIP-seq signal intensity in 42D^ENZR cells and DHT-stimulated LNCaP cells from Jin et al and Zhang et al. The shade of green reflects binding intensity. Each horizontal line represents a 6-kb locus.