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. 2022 Apr 15;13:42. doi: 10.1186/s40104-022-00689-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Beta-actin (ACTB), prohibitin (PHB), heat shock protein family D member 1 (HSPD1), and arginine vasopressin receptor 2 (AVPR2) mRNA expression in high- and low-litter size boar spermatozoa. Differences in marker candidate gene expression in the high-litter size (n = 3) and low-litter size (n = 3) spermatozoa groups based on the average litter sizes. A AVPR2 mRNA expression in boar spermatozoa with high- and low-litter sizes. B ACTB mRNA expression in boar spermatozoa with high- and low-litter sizes. C PHB mRNA expression in boar spermatozoa with high- and low-litter sizes. D HSPD1 mRNA expression in boar spermatozoa with high- and low-litter sizes. Relative expression was normalised to GAPDH expression. The data are expressed as the mean ± standard error of the mean (SEM); ^*P < 0.05