Fig. 4.

Fertility parameters linked to gene expression dysregulation. A Linear regression of HSPD1 expression and motility (%). B Linear regression of HSPD1 expression and curvilinear velocity (μm/s). C Linear regression of HSPD1 expression and average path velocity (μm/s). D Linear regression of HSPD1 expression and mean amplitude of lateral head displacement (μm/s). E Difference of motility in high- and low-HSPD1 expression groups. F Difference of curvilinear velocity (μm/s) in high- and low-HSPD1 expression groups. G Difference of straight-line velocity (μm/s) in high- and low-HSPD1 expression groups. H Difference of average path velocity (μm/s) in high- and low-HSPD1 expression groups. I Difference of wobble (%) in high- and low-HSPD1 expression groups. J Difference of mean amplitude of lateral head displacement (μm/s) in high- and low-HSPD1 expression groups. The average values of each fertility parameter were compared based on the cut-off values of HSPD1 expression (3.1798) from the ROC curves. K Correlation heatmap of all parameters. L Average litter size of high- and low-litter size groups separated by HSPD1 mRNA expression. The data are expressed as the mean ± SEM; ^*P < 0.05