MZ B cell depletion and recovery are rapid. (A–F) Percentage of (A) CD19+ splenic B cells, (B) MZ and (C) MZP B cells, (D) FOL B cells, and (E) Ly6Chi and (F) Ly6Clo monocytes in C57BL/6 mice 24 h after treatment with PBS or a single, 100-µg dose of anti-CD47 Ab. Data are from two independent experiments with 5–10 mice per group. (G–L) Percentage of (G) CD19+ splenic B cells, (H) MZ and (I) MZP B cells, (J) FOL B cells, and (K) Ly6Chi and (L) Ly6Clo monocytes in C57BL/6 mice 24 h after treatment with PBS or anti-CD47 Ab. Spleens were harvested on day 24. Data are from one experiment with three to five mice per group. Groups were compared with an unpaired, two-tailed t test with Welch’s correction. Data are presented as the mean ± SEM. **p < 0.01; ns, not significant.