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. Author manuscript; available in PMC: 2022 Sep 14.
Published in final edited form as: Anal Chem. 2021 Dec 4;93(49):16528–16534. doi: 10.1021/acs.analchem.1c03705

Figure 3.

Figure 3.

Time-dependent increase of fluorescence signals for detection of CXCL9 at 4000 pg/mL by using immuno-CRISPR assay with DNA barcodes and their complexes (a). Calibration curves of immuno-CRISPR assay with SA-DNA barcode-III complex (b) and HRP-based ELISA (c) for detection of CXCL9 at concentrations from 0 to 4000 pg/mL in synthetic urine. Cross-reactivity of the immuno-CRISPR assay using CXCL9 and CXCL1 in crossover experiments. Two different complexes were prepared, anti-CXCL9 antibody conjugated DNA barcode-III complex and anti-CXCL1 antibody conjugated DNA barcode-IV complex (d). Error bars are means and SDs from three independent experiments. Cutoff values based on limit of signal to background absorbance or fluorescence.