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Preparation process of IVT mRNA. (1) cDNA encoding antigen inserted to DNA template. DNA template must include the sequence of the T7 promoter, 5′ UTR, and 3′ UTR. (2) T7 RNA polymerase synthesis IVT mRNA. Both 5′ cap and the poly A tail can be synthesized in two methods. (3) A purification process is required to remove unintended and immunogenic impurities
