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. 2022 Apr 15;3(2):e125. doi: 10.1002/mco2.125

FIGURE 1.

FIGURE 1

PP2A catalyzes the dephosphorylation of XPO5. (A) HEK‐293T cells were transfected with plasmids expressing MEKDD and different catalytic subunits of serine/threonine phosphatases (PP1, PP2B, and PP2A). The phosphorylated and total XPO5/ERK proteins were detected via Western blot. (B) HEK‐293T cells were transfected with plasmids expressing MEKDD, myc‐XPO5 and C subunits of PP2A and myc‐XPO5 was immunoprecipitated from cell lysates. The immunoprecipitates were immunoblotted with the indicated antibodies. (C) The lysates from HEK‐293T cells co‐transfected with the indicated plasmids were subjected to immunoprecipitation via anti‐myc antibody. The enriched complexes were immunoblotted with the indicated antibodies. MEKDD: constitutively active MEK; p‐XPO5 (416): phosphorylated XPO5 at serine 416.