FIGURE 2.

B55β is specifically engaged in XPO5 dephosphorylation. (A) HEK‐293T cells were transfected with plasmids expressing MEKDD and different regulatory subunits. The phosphorylated and total XPO5/ERK proteins were detected via Western blot. (B) SK‐Hep1 cells were transfected with plasmids expressing different regulatory subunits. The phosphorylated and total XPO5 proteins were detected via Western blot and the protein bands were quantified by ImageJ software. Comparison of the intensity of p‐XPO5 bands (using total XPO5 as loading control) was indicated as fold change relative to ctrl set. (C) SK‐Hep1 cells were co‐transfected with the indicated plasmids and myc‐XPO5 was immunoprecipitated from cell lysates. The immunoprecipitates were immunoblotted with the indicated antibodies.