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. 2022 Mar 28;12:844691. doi: 10.3389/fonc.2022.844691

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Copyright © 2022 Tang, Lu, Zhu, Zheng, Fang, Ji and Lu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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To explore the potential mechanism of the risk model-related genes GPI, IL22RA1, CCT6A and SPOCK1 on the progression of LUAD. (A) GSEA analysis was conducted using TCGA_LUAD database to compare the high expression and low expression of GPI, IL22RA1, CCT6A and SPOCK1, respectively. (B) Western blotting analysis of the protein expression level of CCT6A in BEAS-2B, A549 and H1299 cells. (C, D) The effect of CCT6A silencing on colony formation in A549 and H1299. (E, F) The effect of CCT6A knockdown on cell migration was analyzed by wound healing assay in A549 and H1299 (scale bar, 100μm). (G) Western blot analysis of indicated protein expression level. GSEA, Gene Set Enrichment Analysis.