Figure 2. Whsc1 deletion promotes Apc-mutated intestinal tumorigenesis.

(A) Immunostaining for WHSC1 in the small intestines of 2-month-old Apc^min/+ mice. Scale bars: 50 μm (including insets). (B) Kaplan-Meier survival plots for Apc^min/+ and Apc^min/+ Whsc1^IEC–/– mice (n = 11). (C) Representative images of ileum tissues from 4-month-old Apc^min/+ and Apc^min/+ Whsc1^IEC–/– mice. Quantification of tumor numbers and tumor loads is shown (n = 11). Scale bar: 1 cm. (D) H&E-stained sections along the small intestine longitudinal axis in tissues from 3-month-old mice. Scale bars: 2 mm (top) and 100 μm (bottom). (E) Immunostaining in adjacent normal intestine from 4-month-old mice. Quantitation of the indicated cells per crypt is shown. Scale bars: 100 μm and 50 μm (enlarged insets). (F) Immunostaining for the indicated proteins and quantitation in adenoma from 4-month-old mice. Scale bars: 100 μm. (G) Representative images of organoids from Apc^min/+ and Apc^min/+ Whsc1^IEC–/– mice. Quantification of the formation efficiency is shown. Scale bars: 100 μm. Data are presented as the mean ± SEM (C and E–G). *P < 0.05 and **P < 0.01, by log-rank test (B), 2-way ANOVA followed by multiple comparisons (C), and 2-tailed Student’s t test (E–G).