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. 2022 Apr 15;132(8):e151725. doi: 10.1172/JCI151725

Figure 9. The microbiome drives liver leukocyte recruitment by promoting CCL5 signaling in NK1.1+ cells.

Figure 9

(A and B) Mice were treated with broad-spectrum antibiotics or vehicle. CD45+ liver leukocytes were purified by FACS and analyzed by single-cell RNA-Seq as in Figure 7A. (A) Heatmap depicting expression levels of diverse chemokines in each cluster for both treatment groups. (B) Violin plots comparing normalized log expression of Ccl5 in each cluster for both treatment groups. ****P < 0.0001. (C) The total number of hepatic leukocytes was compared in WT versus Ccl5–/– mice. Data are representative of experiments performed 3 times. n = 5/group. *P < 0.05. (D and E) The number of bulk CD45+ (D) and CD45+NK1.1– (E) hepatic leukocytes was compared in WT mice treated with a neutralizing αNK1.1 mAb or isotype control. n = 5/group. *P < 0.05; **P < 0.01.