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. 2022 Apr 15;132(8):e148073. doi: 10.1172/JCI148073

Figure 3. Senolytic drugs enhance fracture healing in aged mice by removing SCs.

Figure 3

Young and aged mice underwent tibial fracture surgery. (A) Experimental outline. Young and aged mice were given 5 mg/kg dasatinib (D) + 50 mg/kg quercetin (Q) or vehicle (V) by gavage on 1, 3, 5, and 7 dpf and sacrificed at 10 or 28 dpf. (B) Expression of p16 and p21 in callus tissues was measured by qPCR at 10 dpf. n = 3. Relative mRNA expression is the fold change versus vehicle-treated young mice as 1. *P < 0.05 vehicle versus D+Q, by 2-way ANOVA followed by Tukey’s post hoc test. (C) Callus volume was measured by micro-CT. n = 3–4. (D) Representative ABH-stained sections showing higher woven bone and callus areas in D+Q-treated mice. Scale bar: 1 mm. (E) Woven bone and cartilage areas were analyzed by ImageJ. n = 3–4. (F) Bone stiffness, strength, and toughness were assessed by biomechanical testing at 28 dpf. n = 6. *P < 0.05 vehicle versus D+Q treatment; #P < 0.05, for D+Q-treated aged mice versus vehicle-treated young mice; 2-way ANOVA followed by Tukey’s post hoc test (C, E, and F). μCT, micro-CT.