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Activation of the ERK, p38, and JNK mitogen-activated protein kinases by treatment with PABA/NO. A, cells were treated with 30 μM PABA/NO for the indicated times. B, cells were treated with the indicated concentrations of PABA/NO for 60 min. 20 μg of protein was loaded in each lane. The protein expression levels of ERK, p38, and JNK were unchanged with the addition of PABA/NO (bottom). Even loading of protein was confirmed by probing for actin (data not shown).
