TABLE 2.
Kinetics of monocyte fluoroquinolone transporta
| Fluoroquinolone | Km (μg/ml) | Vmax (ng/min/106 cells) | Vmax/Km |
|---|---|---|---|
| Ciprofloxacin | 171* ± 8.27 | 32.7† ± 4.68 | 0.190 ± 0.034 |
| Norfloxacin | 174* ± 16.4 | 33.8† ± 1.64 | 0.198 ± 0.015 |
| Ofloxacin | 297 ± 21.8 | 84.6* ± 10.8 | 0.286 ± 0.040 |
| Lomefloxacin | 537 ± 26.8 | 92.3* ± 10.0 | 0.173 ± 0.018 |
a
Monocytes were suspended in HBSS and incubated with various concentrations of ciprofloxacin for 4 min. The assays were terminated by pelleting the cells through an oil cushion. Km and Vmax values were determined by Lineweaver-Burk analysis. Results are expressed as the mean and standard error of the mean of four determinations. The differences in mean Km and Vmax values were greater than would be expected by chance (P < 0.05, ANOVA). Identical superscripts within a column denote pairwise comparisons that were not significantly different (P > 0.05, Tukey's test). Significant differences were noted in all other comparisons.