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. Author manuscript; available in PMC: 2022 Apr 15.
Published in final edited form as: Cell Rep. 2019 Dec 3;29(10):2944–2952.e5. doi: 10.1016/j.celrep.2019.10.115

Figure 3. RhoGEF Signaling Is Required for NLGN1 Overexpression-Mediated Spine Formation.

Figure 3.

(A) Confocal images of cultured hippocampal neurons transfected at DIV5 with a combination of GFP, NLmiRs, HA-NLGN1, miR-KAL (Kalirin knockdown (KD), and miR-KAL and TRIO-shRNA (Kalirin/Trio KD). Cells were stained for GFP and HA and imaged at DIV12–DIV14. Scale bar is 20 μm.

(B) Spine number quantification of (A), with a one-way ANOVA and Tukey’s multiple comparison test across independent conditions (n = 3). ±SEM is reflective of total number of observations.