KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Rabbit Kalirin [1:1000] | Millipore | Cat#: 07–122 RRID:AB_310380 |
| Rabbit HA [1:2000] | Abcam | Cat#: ab9110 RRID:AB_307019 |
| Rabbit Myc (71D10) [1:5000] | Cell Signaling | Cat#: 2278 |
| Mouse Actin [1:5000] | Abm | Cat#: G043 |
| Rabbit IgG | Millipore | Cat# PP64 RRID:AB_97852 |
| Rabbit Neuroligin-3 [1:1000] | Synaptic Systems | Cat#: 129103 RRID:AB_887748 |
| Mouse Neuroligin-1 [1:1000] | Synaptic Systems | Cat#: 129111 RRID:AB_887747 |
| Mouse Neuroligin-2 [1:2000] | Synaptic Systems | Cat#: 129511 RRID:AB_2619813 |
| Rabbit Trio [1:1000] | Abcam | Cat#: ab194365 RRID:AB_11127474 |
| Mouse Dpysl2 (Crmp2) [1:1000] | Abcam | Cat#: ab62539 RRID:AB_941175 |
| Mouse Gephyrin [1:1000] | Cell Signaling | Cat#: 14304 RRID:AB_2798443 |
| Rabbit Kalirin-7 [1:3000] | This manuscript | Cat#: N/A |
| Rabbit Trio [1:1000] | This manuscript | Cat#: N/A |
| Rabbit Trio [1:1000] | This manuscript | Cat#: N/A |
| Mouse GluN2A [1:1000] | Sigma-Aldrich | Cat#: SAB5200888 RRID:AB_2629501 |
| Mouse GluN2B [1:1000] | Neuromab | Cat#: Q00960 |
| Mouse GluA1 [1:1000] | Antibodies Inc | Cat#: 75327 RRID:AB_2315840 |
| Rabbit GFP [1:1000] | Thermo Fischer | Cat# A11122 RRID:AB_221569 |
| Mouse IgG HRP linked whole antibody [1:5000] | GE Healthcare | Cat#: NA931 RRID:AB_772210 |
| Rabbit IgG HRP linked whole antibody [1:5000] | GE Healthcare | Cat#: NA934 RRID:AB_772206 |
| Anti-mouse IgG (H+L) Alexa Fluor 488 [1:500] | Thermo Fisher | Cat#: A21131 RRID:AB_141618 |
| Anti-mouse IgG (H+L) Alex Fluor 555 [1:500] | Thermo Fisher | Cat#: A21422 RRID:AB_141822 |
| Anti-rabbit IgG (H+L) Alexa Fluor 555 [1:500] | Thermo Fisher | Cat#: A21428 RRID:AB_141784 |
| Anti-rabbit IgG (H+L) Alexa Fluor 488 [1:500] | Thermo Fisher | Cat#:A11034 RRID:AB_2576217 |
| Nanogold®-Fab’ Goat anti-Mouse IgG (H+L) | Nanoprobes Inc. | Cat #2001 |
| Nanogold®-IgG Goat anti-Rabbit IgG (H+L) | Nanoprobes Inc. | Cat #2003 |
|
| ||
| Chemicals, Peptides, and Recombinant Proteins | ||
|
| ||
| Lipofectamine 2000 | Thermo Fisher | Cat# 11668019 |
| Protease inhibitor mixture | Roche | Cat#: 11697498001 |
| Phosphatase inhibitor mixture II | Sigma-Aldrich | Cat#: P5726 |
| Phosphatase inhibitor mixture III | Sigma-Aldrich | Cat#: P0044 |
| Glutathione Sepharose 4B | GE Healthcare | Cat#: 17075601 |
| Protein A-Sepharose | Sigma-Aldrich | Cat#: P3391 |
|
| ||
| Cell Lines | ||
|
| ||
| Human embryonic kidney (HEK) 293T | ATTC | Cat#: CRL11268 RRID:CVCL_1926 |
| Escherichia coli: BL21(DE3) | Agilent | Cat#: 200132 |
|
| ||
| Recombinant DNA | ||
|
| ||
| pCAG-Trio-9 | Herring and Nicoll, 2016 | N/A |
| pCAG-HA–Nlgn1 | Bemben et al., 2014 | N/A |
| IRES-HA–Nlgn1 | Bemben et al., 2014 | N/A |
| pEAK10-His-Myc-Kalirin-5 | Addgene | Cat#: 25440 RRID:Addgene_25440 |
| pEAK10-His-Myc-Kalirin-7 | Addgene | Cat#: 25454 RRID:Addgene_25454 |
| pEAK10-His-Myc-Kalirin-9 | Addgene | Cat#: 25441 RRID:Addgene_25441 |
| pEAK10-His-Myc-Kalirin-12 | Addgene | Cat#: 25442 RRID:Addgene_25442 |
| pEAK10-Myc-Sec14pSR1:4 | Dr. Betty Eipper (UConn) | N/A |
| pGEX-Sec14p | Dr. Betty Eipper (UConn) | N/A |
| pCAG-NLmiRs-GFP | Bemben et al., 2014 | N/A |
| pCAG-eGFP | Bemben et al., 2014 | N/A |
| Kalirin-miR & Trio shRNA | Herring and Nicoll, 2016 | N/A |
| pCAG-Kalirin7 | Herring and Nicoll, 2016 | N/A |
|
| ||
| Organisms/Strains | ||
|
| ||
| Sprague Dawley® outbred rat | Envigo | Cat#: 002 |
| C57BL/6 inbred mouse | Charles River | Cat#: 027 |
| NLGN1 −/− | Bemben et al., 2014 | N/A |
|
| ||
| Software and Algorithms | ||
|
| ||
| Adobe Photoshop CC 2018 | Adobe | RRID:SCR_014199 |
| Adobe Illustrator CC 2018 | Adobe | RRID:SCR_010279 |
| ImageJ | NIH | RRID:SCR_003070 |
| GraphPad Prism 8 | Graphpad | RRID:SCR_002798 |
| Proteome Discoverer 2.2 | Thermo Fisher | Cat# 30795 RRID:SCR_014477 |
| Cytoscape | Cytoscape | RRID:SCR_003032 |