Figure 2.

Characterizing D1 alveolar epithelium cell states

(A) UMAP of annotated D1 alveolar epithelial clusters.

(B) Violin plots of gene activity levels for notable AT1.AT2 cell differentially accessible genes.

(C) Immunofluorescent LIMD1, AGER, and SFTPC staining in alveolar region of human in D1, Y3, and adult lung from different donors. White arrows indicate triple positive AT1.AT2 cells. Yellow arrows indicate AGER and LIMD1 in AT1 cells. scTHS-seq data collected from donor 1 in each age group except for adult sample. Scale bars units listed in bottom left corner for each image. See also Figure S3D.

(D) Dot plot of AT1.AT2 marker gene expression in AT1/AT2-like cells from Wang et al. snRNA-seq grouped by donor age, ∼30 weeks gestational age and 3 and 31 years old.

(E) GREAT significant GO biological processes for D1 AT1 clusters for top 2k differential peaks based on -log₁₀(binomal p value)⁴³.

(F) Dot plot of gene activity scores for AT1 marker genes in alveolar epithelial clusters.

See also Figure S3 and Table S4.