Figure 5.

Exploring myofibroblast states at D1 and epithelial-mesenchymal regulatory modules guiding alveolar septation

(A) UMAP of annotated D1 AT1.AT2, AT1 and myoFB clusters.

(B) Dot plot of log-normalized gene activity scores for PDGFA and select myoFB DA genes.

(C) UMAP from Figure S3A re-colored with TF activity levels for key myoFB TFs.

(D) Bar plots of -log₁₀(p values) for select active genes in myoFB_1 or myoFB_2 cells with significant NFATC, MEF2, and SRF motif enrichment.

(E) Dot plot of TF gene expression in Wang et al. snRNA-seq by myoFBs grouped by BRINDL donor age, ∼30 weeks gestational age, 3, and 31 years old.

(F) Dot plot of active gene expression in Wang et al. snRNA-seq grouped by donor age.

(G) Proposed regulatory modules for D1 PDGFA:PDGFRA signaling and myoFB differentiation. All marker genes in rectangles contained peaks enriched in TF motifs and are expressed in AT1 or myoFB clusters in Wang et al. sn RNA-seq at ∼30 week D1.

See also Table S4.