Figure 6.
Divergent PIEZO2 mechanotransduction signaling in fibroblasts at postnatal day 1 and across development
(A–D) PIEZO2 gene activity and expression at D1 and P1. Log-normalized gene activity from all D1 (A) and P1 (C) cell types. Average expression in transcripts per million (TPM) from LGEA scRNA-seq database for D1 (B) and P1 (D) lung.
(E–F) Connectivity plots for human and mouse open chromatin regions linked to PIEZO2 and genome browser tracks with peaks highlighted for collapsed cell-type bigwigs. Height of connections represent Cicero co-accessibility score between connected peaks.
(G) PIEZO2 gene activity for all human matrix fibroblasts grouped by age.
(H) Immunofluorescent staining for PIEZO2 and ACTA2 in alveolar region of human in D1, Y3, and adult lung from different donors. scTHS-seq data collected from donor 1 in each age group except for adult sample. Scale bars units listed in bottom left corner for each image. See also Figure S7C.
(I) Dot plot of average expression of active genes in LGEA D1 scRNA-seq dataset.
(J) Proposed regulatory module for D1 MFB_1. All marker genes in rectangles contained peaks enriched in NHLH2 motifs and are expressed in MatrixFB1 cluster in LGEA D1 human.