REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PIEZO2 Antibody (Rabbit polyclonal)	Invitrogen	Cat#PA5-56894; RRID: AB_2645539
Alpha smooth muscle actin Antibody (Mouse/IgG2a, kappa)	Invitrogen	Cat#14-9760-82; RRID: AB_2572996
Human LIMD1 Antibody (Mouse monoclonal IgG)	R&D Systems	Cat#MAB8494; Clone#919330; Accession#Q9UGP4
Wang et al., 2020 snRNA-seq	Wang et al., 2020	GEO: GSE161382
Human molecular cell atlas	Travaglini et al., 2020	EGA accession: EGAS00001004344
Alexa Fluor Plus 555 (Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody)	Invitrogen	Cat#A32816; RRID: AB_2762839
DyLight 755 (Donkey anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody)	Invitrogen	Cat#SA5-10043; RRID: AB_2556623
Alexa Fluor 647 (Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody)	Invitrogen	Cat#A32787; RRID: AB_2762830
Biological Samples
Frozen human lung biopsies and fresh frozen paraffin-embedded sections	Biorepository for Investigation of Neonatal Diseases of the Lung (BRINDL)	https://brindl.urmc.rochester.edu/
Fresh frozen paraffin-embedded unused adult deceased donor lung tissue	University of California San Diego (UCSD)	N/A
Chemicals, Peptides, and Recombinant Proteins
100x Citrate Buffer pH 6.0	Abcam	Cat#ab64236
VECTASHIELD Vibrance Antifade Mounting Medium with DAPI	Vector Laboratories	Cat#H-1800
Deposited Data
Raw data	This paper	GEO: GSE154027
Cellular census of human lungs	Vieira Braga et al., 2019	GEO: GSE130148
Cell atlas of mouse lung development	Cohen et al., 2018	GEO: GSE119228
scRNA-seq mouse lung at birth	Guo et al., 2019	GEO: GSE122332
LGEA scRNA-seq	Du et al., 2015; Du et al., 2017; Du et al., 2021	www.lungmap.net
Experimental Models: Organisms/Strains
C57BL/6 mice frozen whole lungs	Perl Lab	N/A
Oligonucleotides
(See Lake et al., 2018 Table S12)	Lake et al., 2018	N/A
Software and Algorithms
Code for processing scTHS-seq data	Lake et al., 2018	https://github.com/yanwu2014/scTHS-Seq-processing
Code for analyzing lung scTHS-seq data	This paper	https://github.com/ThuElizabethD/Lung-scTHS-seq
bcl2fastq	Github	https://github.com/brwnj/bcl2fastq
deindexer	Github	https://github.com/ws6/deindexer
BWA	Github	https://github.com/lh3/bwa
pysam	Github	https://github.com/pysam-developers/pysam
SAMtools	Danecek et al., 2021	https://www.htslib.org/
SnapATAC	Fang, Priessl et al., 2021	https://github.com/r3fang/SnapATAC
MACS	Github	https://github.com/macs3-project/MACS
cisTopic	Bravo González-Blas et al., 2019	https://github.com/aertslab/cisTopic
Seurat	Stuart, Butler et al., 2019	https://satijalab.org/seurat/
Cicero	Pliner et al., 2018	https://cole-trapnell-lab.github.io/cicero-release/
deepTools	Ramirez et al., 2016	https://github.com/deeptools/deepTools
ChromVAR	Schep et al., 2017	https://greenleaflab.github.io/chromVAR/index.html
Signac	Stuart et al., 2021	https://satijalab.org/signac/
ChromVARmotifs	Github	https://github.com/GreenleafLab/chromVARmotifs
ChIPseeker	Guangchuang et al., 2015	http://bioconductor.org/packages/release/bioc/html/ChIPseeker.html
GViz	Hahne and Ivanek, 2016	https://bioconductor.org/packages/release/bioc/html/Gviz.html
TxDb.Hsapiens.UCSC.hg38.knownGene R package v3.4.6	Bioconductor	https://doi.org/10.18129/B9.bioc.TxDb.Hsapiens.UCSC.hg38.knownGene
TxDb.Mmusculus.UCSC.mm10.knownGene R package v3.4.7	Bioconductor	https://doi.org/10.18129/B9.bioc.TxDb.Mmusculus.UCSC.mm10.knownGene
org.Hs.eg.db R package v3.8.2	Bioconductor	https://doi.org/10.18129/B9.bioc.org.Hs.eg.db
org.Mm.eg.db R package v3.8.2	Bioconductor	https://doi.org/10.18129/B9.bioc.org.Mm.eg.db
g-chromVAR	Ulirsch et al., 2019	https://github.com/caleblareau/gchromVAR
CausalDB fine-mapping pipeline	Github	https://github.com/mulinlab/CAUSALdb-finemapping-pip
R/Rstudio	R/Rstudio	https://rstudio.com/
Other
GentleMACS Dissociator	Miltenyi Biotec	Cat#130-093-235
GentleMACS C Tubes	Miltenyi Biotec	Cat#130-093-237
Sterile single-pack CellTrics filters - 30 um	CellTrics	Cat#04-004-2326
S2002 Dako Pen	Agilent Technologies	Cat#S200232-3
scTHS-seq Assay	Lake et al., 2018	https://doi.org/10.17504/protocols.io.xf8fjrw
TC20 Automated Cell Counter	Bio-Rad	Cat#1450102
HiScribe T7 High Yield RNA Synthesis Kit	New England BioLabs	Cat#E2040S
Taq 5X Master Mix	New England BioLabs	Cat# M0285L
Taq DNA Polymerase with Standard Taq Buffer	New England BioLabs	Cat#M0273E
AMPure XP	Beckman	Cat#A63882
Clontech SMART MMLV reverse transcriptase kit	Takara	Cat#639523
RNase H	Enzymatics	Cat#Y9220L