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AT III abrogates LPS-induced epithelial barrier impairment in IEC-6 cells. (a) Paracellular permeability was measured using FITC-dextran. (b, c) Western blots for occludin and ZO-1 proteins and quantification results. (d, e) Representative images of occludin were determined and quantified by immunofluorescence. (f, g) Representative images of ZO-1 were measured and quantified by immunofluorescence. Scale bar: 50 μm. $p < 0.05, compared to control; &p < 0.05, compared to LPS.
