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. Author manuscript; available in PMC: 2023 Apr 15.
Published in final edited form as: J Immunol. 2022 Mar 30;208(8):2029–2036. doi: 10.4049/jimmunol.2101154

FIGURE 4.

FIGURE 4.

NEK9 knockdown fails to restore defective NLRP3 inflammasome activation caused by NEK7 R121Q mutation. (A) HA-tagged NEK7, NEK7 R121Q, NEK6, or NEK6Q132R was co-expressed with NEK9 in HEK 293T cells. Cell lysates were immunoprecipitated with an anti-HA antibody and immunoblotted with indicated antibodies. (B) Parent (WT) or NEK7R121Q knock-in mutant (KI) macrophages were treated with control shRNA or two individual NEK9-targeting shRNAs. Macrophages were stimulated with PBS or 5 μM nigericin after LPS priming. Cell lysates were immunoblotted with indicated antibodies. (C) Measurement of IL-β in the supernatants from PBS or nigericin-stimulated macrophages after the treatment of indicated shRNAs by ELISA. Data are representative of three experiments. Error bars in C show the SEM of triplicate wells.