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. Author manuscript; available in PMC: 2022 Oct 8.
Published in final edited form as: Nat Methods. 2022 Apr 8;19(4):449–460. doi: 10.1038/s41592-022-01436-z

Figure 3 |. LeAPS-based lentiviral packaging enables scalable viral library construction.

Figure 3 |

A, Schematic overview of the limitation of conventional lentiviral packaging strategies for surface displayed lentiviral libraries. B, Schematic overview of the mechanism of promoter translocation used in the LeAPS system. C, Packaging cells transduced with viruses containing LeAPS-encoding genomes and then transfected with lentiviral helper plasmids (top), and transduced with viruses containing standard genomes (bottom). D, LeAPS-produced viruses maintain TCR specificity in two independent TCR-pMHC systems,. E, Schematic overview of the implementation of LeAPS for viral library assembly. F, On-target enrichment of libraries using defined mixtures of A2-NY-ESO-1 and A2-SL9 packaging cells to represent 100- or 1,000-variant libraries.