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. Author manuscript; available in PMC: 2022 Oct 8.
Published in final edited form as: Nat Methods. 2022 Apr 8;19(4):449–460. doi: 10.1038/s41592-022-01436-z

Extended Data Fig. 3. Schematic diagram of LeAPS proof of concept experiments.

Extended Data Fig. 3

Packaging cells transduced with (i) SL9 presented on HLA-A2 and LeAPS-mCherry and (ii) NY-ESO-1 presented on HLA-A2 and LeAPS-GFP were mixed at either 1:100 or 1:1000 for each to represent libraries containing 100 constructs and 1,000 constructs, respectively. These mixtures were then used as packaging lines, transfected with helper plasmids to produce viruses, and used to transduce mixtures of cell tracking dye-labeled J76 cells expressing either 868 TCR or 1G4wt TCR at defined ratios.