FIGURE 5.

Il27ra^−/− mice have decreased presence of γδ T cells in the alveolar space during N. brasiliensis infection.

(A) Representative flow cytometry gating of TCRβ⁻TCRγδ⁺ γδ T cells (top panels), CD4⁺CD8⁻ Th cells, and CD4⁻CD8⁺ CTLs (bottom panels), from WT control (C57BL/6NJ) (left panels) and Il27ra^−/− mice (right panels) in BAL during N. brasiliensis infection (n=500 L3/mouse, s.c.) on day 2 p.i. Frequencies (%) of parent populations are indicated next to each gate. Cells were pre-gated on live singlet lymphocytes. (B) Frequencies and (C) absolute numbers of these CD4⁺CD8⁻ Th cells, CD4⁻CD8⁺ CTLs, and TCRβ⁻TCRγδ⁺ γδ T cells in BAL of WT (n=5 mice/group; one mouse was removed due to extremely low infection, as determined by negligible hemorrhage) and Il27ra^−/− (n=6 mice/group) mice on day 2 p.i. Each circle indicates an individual mouse (B, C). Data are shown as mean ± SEM (B, C) and were analyzed by Student’s t-test (B,C), * P<0.05, ns: not significant.