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. 2022 Apr 15;12:6356. doi: 10.1038/s41598-022-09855-w

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© The Author(s) 2022, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Pearson correlation coefficients between the results of 16S rRNA amplicon-seq and PICRUSt2 in the ruminal bacterial composition. The taxonomy_7_levels.txt file in the SILVA database was used for annotation of bacterial OTUs in the amplicon-seq data. The color of the DE taxa indicates a statistically significant difference among the groups (q < 0.01) analyzed by TCC baySeq. PICRUSt2 analysis was performed with dada2 output in QIIME2. The MetaCyc pathway was searched in the PICRUSt2 pipeline. Correlations with a coefficient between 0.7 and 1.0 are shown. Aromatic compound degradation and cofactor, carrier, and vitamin biosynthesis are shown as filled circles and triangles, respectively.