Skip to main content
. 2022 Apr 15;13(4):353. doi: 10.1038/s41419-022-04767-z

Fig. 3. REV-ERBα agonism impairs autophagic flux in β-cells.

Fig. 3

A INS-1E cells expressing mCherry-EGFP-LC3B plasmid were either cultured in the absence or presence of lysosomal inhibitors (LI: E-64-d, 10 μg/ml and pepstatin A, 10 μg/ml, 24 h), or exposed to REV-ERB agonist (SR9009, 10 μM, 24 h) or control (DMSO). B Autophagic flux was assessed by quantification of mCherry and EGFP puncta colocalization using the Image J software. Results are expressed as the percentage of mCherry and EGFP colocalization per cell (top graph), and as fold change vs. control (bottom graph). Data are expressed as mean ± SEM of three to four independent experiments; *P < 0.05, **P < 0.01 vs. control.