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. 2022 Apr 15;13(4):353. doi: 10.1038/s41419-022-04767-z

Fig. 4. REV-ERBα agonism impairs autophagic flux in human islets.

Fig. 4

A Human islets were exposed to REV-ERB agonist (SR9009, 20 μM, 72 h) or control (DMSO). Protein levels of p62, high molecular weight (HMW)-p62, Cleaved Caspase-3, and actin (loading control) were analyzed by western blot. B Graphs represent the quantification of the blots (n = 4 independent experiments). Data are expressed as mean ± SEM; *P < 0.05 vs. control. C p62 staining was assessed by immunofluorescence (p62, green; insulin, red; nuclei, blue) in paraffin-embedded human islets exposed to REV-ERB agonist (SR9009, 20 μM, 72 h) or control (DMSO). D Dispersed human islets cells were exposed to REV-ERB agonist (SR9009, 20 μM, 24 h) or control (DMSO), and then fixed for immunofluorescence detection of p62 (red), insulin (green) and nuclei (blue). p62-positive cytoplasmic inclusions in β-cells are indicated with arrowheads. Scale bar: 5 μm.