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. 2022 Apr 15;13(4):353. doi: 10.1038/s41419-022-04767-z

Fig. 6. Negative modulation of REV-ERBα protects β-cells and human islets from cytokines-induced apoptosis.

Fig. 6

INS-1E cells (A) or human islets (B) were exposed or not to pro-inflammatory cytokine mix (CK) in the presence or absence of REV-ERB antagonist (SR8279, 5 or 10 μM for 24 h). Cleaved Caspase-3 and actin (loading control) levels were analyzed by western blot (n = 3–4 independent experiments). C REV-ERBα was silenced in INS-1E cells by siRNA. Scramble RNA (Scr) was used as a control. Cells were then exposed to pro-inflammatory cytokine mix (CK). Levels of REV-ERBα, Cl. Caspase-3, and actin were analyzed by western blot (n = 3 independent experiments). For REV-ERBα and Cl. Caspase-3 images, lanes were run on the same gel but were non-continuous (as indicated). Data are expressed as mean ± SEM; *P < 0.05 vs. control.