Skip to main content
. 2022 Apr 15;8:205. doi: 10.1038/s41420-022-00932-7

Fig. 3. miR-15a-5p promotes apoptosis and represses the proliferation of CCA cells by targeting CHEK1.

Fig. 3

A Western blot assay of CHEK1 protein in HuCCT1 and HuH28 cells. Quantitative results of protein bands are displayed at the top of the panel. B Proliferative analysis of HuCCT1 and HuH28 cells. OD value was assessed at 450 nm. C Apoptosis evaluation of HuCCT1 and HuH28 cells using flow cytometry. In panels A–C miR-15a-5p mimic, miR-15a-5p mimic + oe-CHEK1, si-CHEK1, and their corresponding controls were individually transfected into HuCCT1 and HuH28 cells. The data are presented as mean ± standard deviation. The values at multiple groups were compared using one-way ANOVA followed by Tukey’s post-hoc tests; *means comparison against the mimic-NC group, P < 0.05; #means comparison against miR-15a-5p mimic + CHEK1-NC group, P < 0.05; &means comparison against the si-NC group, P < 0.05. Cell experiments were repeated three times independently.