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. 2022 Apr 15;8:205. doi: 10.1038/s41420-022-00932-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Flow Cytometry detection of cell surface markers of HUCMSCs. B Observation of cell morphology and identification of differentiation-capacity under an inverted microscope. From left to right: Cell morphology was observed under an inverted microscope, bar = 50 μm; differentiation of osteoblasts was confirmed using Alizarin Red staining, bar = 25 μm; differentiation of adipocytes was assessed using Oil Red staining, bar = 25 μm; differentiation of chondroblasts was identified using Alcian Blue staining, bar = 25 μm. C TEM evaluation of exosome morphology. Bar = 100 μm. D NTA of particle size distribution of exosome. E Western blot assay of expression of the exosomal marker using ALIX, CD63 and TSG101 antibodies; calnexin antibody served as a NC. F Quantitative amplified results of level of miR-15a-5p in HUCMSCs and HUCMSCs-exo. Comparison to the control group was conducted using independent sample t-test; *means comparison against HUCMSCs, P < 0.05. The data are presented as mean ± standard deviation. Cell experiments were repeated three times independently.