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. 2022 Apr 15;8:205. doi: 10.1038/s41420-022-00932-7

Fig. 8. miR-15a-5p in HUCMSCs-exo promotes apoptosis and represses the proliferation of CCA cells via downregulation of CHEK1.

Fig. 8

A miR-15a-5p expression in HUCMSCs-exo determined by RT-qPCR. HUCMSCs-exo was sourced from HUCMSCs manipluated with mimic-NC, miR-15a-5p mimic, inhibitor-NC, or miR-15a-5p inhibitor. B miR-15a-5p expression in HuCCT1 and HuH28 cells determined by RT-qPCR. C Western blot assay of CHEK1 protein in HuCCT1 and HuH28 cells. D Proliferative analysis of HuCCT1 and HuH28 cells. OD value was assessed at 450 nm. E Apoptosis evaluation of HuCCT1 and HuH28 cells using flow cytometry. B–E Exo-miR-15a-5p mimic or Exo-miR-15a-5p inhibitor was individually transfected into CCA cells. The values at multiple groups were compared using one-way ANOVA followed by Tukey’s post-hoc tests; *means comparison against Exo-mimic-NC group, P < 0.05; #means comparison against Exo-inhibitor-NC group, P < 0.05. The data are presented as mean ± standard deviation. Cell experiments were repeated three times independently.