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. 2022 Apr 15;8:203. doi: 10.1038/s41420-022-00896-8

Fig. 2. Quantification of SIRT1 expression and inflammatory factor production in patients with OA.

Fig. 2

A qRT-PCR to detect the expression of SIRT1 mRNA in normal cartilage tissue (n = 10) and OA cartilage tissue (n = 20). B Western blot to detect the expression of SIRT1 protein in normal cartilage tissue (n = 10) and OA cartilage tissue (n = 20). C ELISA to detect the level of IL-1β in serum of OA patients (n = 20). D ELISA to detect the level of TNF-α in serum of OA patients (n = 20). **p < 0.01 compared with Ctrl group.