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. 2022 Apr 15;8:203. doi: 10.1038/s41420-022-00896-8

Fig. 3. Characterization of SIRT1 expression in IL-1β-stimulated chondrocytes in vitro.

Fig. 3

A Microscopic image of primary human chondrocytes. B Toluidine blue staining to detect Proteoglacan expression in human chondrocytes in response to IL-1β stimulation. C Immunofluorescence staining assay to detect Collagen II expression (red fluorescence) in human chondrocytes in response to IL-1β stimulation. D ELISA to detect TNF-α and MMP-13 in the cell supernatant of primary human chondrocytes in response to IL-1β stimulation. E Immunofluorescence staining for the expression of Aggrecan and Collagen II in human chondrocytes in response to IL-1β stimulation. F Western blot for the protein expression of Collagen II and Aggrecan in primary human chondrocytes in response to IL-1β stimulation. G qRT-PCR detection of SIRT1 in primary human chondrocytes in response to IL-1β stimulation. H Western blot detection of SIRT1 protein expression in primary human chondrocytes in response to IL-1β stimulation. **p < 0.01 compared with Ctrl group. Cell experiments were performed in triplicates.