Skip to main content
. 2022 Apr 15;8:203. doi: 10.1038/s41420-022-00896-8

Fig. 4. SIRT1 overexpression reduces EGFR ubiquitination by suppressing PTEN expression.

Fig. 4

A qRT-PCR detection of PTEN and EGFR expression in normal (n = 10) and OA (n = 20) cartilage tissues. B Western blot detection of PTEN and EGFR protein expression in normal (n = 10) and OA (n = 20) cartilage tissues. C qRT-PCR detection of SIRT1 transduction efficiency in IL-1β-challenged primary human chondrocytes. D Western blot detection of expression levels of SIRT1, PTEN and EGFR proteins in IL-1β-challenged primary human chondrocytes after transduction with oe-SIRT1 or sh-SIRT1. E Immunoprecipitation assay of acetylation of PTEN in IL-1β-challenged primary human chondrocytes after oe-SIRT1 or sh-SIRT1 treatment. F qRT-PCR measurement of PTEN expression in response to sh-PTEN treatment. G Western blot detection of PTEN and EGFR protein expression in IL-1β-challenged primary human chondrocytes after transduction with sh-PTEN. H Detection of EGFR ubiquitination caused by EGF-stimulated chondrocytes after knockdown of PTEN. **p < 0.01 compared with the Ctrl group. ##p < 0.01 compared with IL-1β group. &&p < 0.01 compared with IL-1β + sh-NC group. %%p < 0.01 compared with IL-1β + oe-NC group.