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. 2022 Apr 15;8:203. doi: 10.1038/s41420-022-00896-8

Fig. 5. SIRT1 affects chondrocyte synthesis, metabolic imbalance and autophagy in OA by upregulating EGFR via PTEN.

Fig. 5

A Safranin O staining to detect the deposition of GAGs in human chondrocytes in the presence of SIRT1 knockdown or restoration. B Western blot to detect p-Akt and Akt protein expression levels in human chondrocytes in the presence of SIRT1 knockdown or restoration. C Western blot for Collagen II, Aggrecan and MMP-13 in human chondrocytes in the presence of SIRT1 knockdown or restoration. D Immunofluorescence staining assay for Collagen II (green fluorescence) and Aggrecan (red fluorescence) after knockdown or restoration of SIRT1. E Western blot assay to detect the expression levels of Beclin-1, LC3B-I, LC3B-II and p62 proteins in human chondrocytes after knockdown or restoration of SIRT1. Cell experiments were performed in triplicates.