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. 2022 Apr 15;12:6261. doi: 10.1038/s41598-022-10143-w

Figure 1.

Figure 1

Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 and MDA-MB-231) and non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, and anti-β-actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 and MDA-MB-231) and non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 and MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody and visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker and the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence and absence of 20 mM lactate for 48 h, and cell lysates were analyzed by immunoblotting using anti-GPR81 and anti-β-actin antibodies.