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. 2022 Apr 15;12:6261. doi: 10.1038/s41598-022-10143-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Impaired ATP production and glycolysis in shGPR81 MDA-MB-231 cells. (a) ShNT and shGPR81 #1 cells were cultured for 24 h with or without 10 mM lactate and intracellular ATP concentrations were measured using an ATP assay kit (n = 3/group). **p < 0.01 using one-way analysis of variance followed by Tukey’s post hoc test. (b) Western blot analysis of glycolytic enzymes in shNT and shGPR81 #1 cells. Cell lysates from shNT and shGPR81 #1 cells were analyzed by immunoblotting using anti-HK2, anti-PFK1, anti-LDHA, and anti-β-actin antibodies.