TABLE 2.
Origin and phenotypic and genotypic characteristics of 11 S. pneumoniae isolates requiring MICs of ≥4 μg/ml for any fluoroquinolone tested
| Isolate | Statea | MIC (μg/ml) (interpretive categoryb) of:
|
MDR phenotypec | Molecular PFGE type | Amino acid alterations in:
|
|||||
|---|---|---|---|---|---|---|---|---|---|---|
| Sparfloxacin | Levofloxacin | Grepafloxacin | Trovafloxacin | Moxifloxacin | GyrA | ParC | ||||
| 1 | CA | 4 (R) | 8 (R) | 4 (R) | 1 (S) | 2 (I) | Yes | A | Ser-81-Phe | Lys-137-Asn |
| 2 | HI | >4 (R) | >8 (R) | >4 (R) | 4 (R) | 4 (R) | No | D | WTd | Ser-16-Gly; Ser-79-Phe; Asn-91-Asp; Glu-125-Asp |
| 3 | HI | >4 (R) | >8 (R) | >4 (R) | 4 (R) | 2 (I) | Yes | A | Ser-81-Phe | Ser-79-Phe; Lys-137-Asn |
| 4 | IL | 2 (R) | >8 (R) | 1 (I) | 1 (S) | 2 (I) | No | E | Ser-81-Tyr | WT |
| 5 | MA | 4 (R) | 8 (R) | 4 (R) | 4 (R) | 4 (R) | No | B | Ser-81-Phe | Ser-79-Phe |
| 6 | MN | >4 (R) | >8 (R) | >4 (R) | 2 (I) | 2 (I) | Yes | G | Ser-81-Phe | Ser-79-Phe |
| 7 | MN | 4 (R) | >8 (R) | 4 (R) | 2 (I) | 2 (I) | Yes | A | Ser-81-Phe | Ser-79-Phe; Lys-137-Asn |
| 8 | NJ | 2 (R) | 4 (I) | 1 (I) | 0.25 (S) | 1 (S) | No | F | Ser-81-Phe | WT |
| 9 | PA | 4 (R) | >8 (R) | 4 (R) | 2 (I) | 2 (I) | Yes | A | Ser-81-Phe | Ser-79-Phe; Lys-137-Asn |
| 10 | VA | >4 (R) | 8 (R) | >4 (R) | 2 (I) | 4 (R) | Yes | H | Ser-81-Tyr | Ser-79-Tyr |
| 11 | VA | 1 (S) | 8 (R) | 1 (I) | 0.25 (S) | 1 (S) | No | C | Ser-81-Phe | Ser-79-Phe; Lys-137-Asn |