Figure 2.

Complex nerve network formed by a single nerve stump in a multibranch nerve conduit. (a) Digital patterns of the multibranch nerve conduit. (b) 3D-printed multibranch nerve conduit (scale bar: 0.2 cm). (c) The 3D-printed multibranch nerve conduit was implanted in rats, with the upper part sutured with the tibial nerve and the lower part embedded in the adjacent muscle; scale bar = 0.2 cm. (d) Successful reconstruction of the complicated nerve network in the multibranch nerve conduit, scale bar = 0.25 cm. Representative Luxol fast blue staining images of the regenerated main nerve (e) and branch nerve (f) (scale bar = 50 μm)