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. 2022 Apr 15;10:tkac010. doi: 10.1093/burnst/tkac010

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© The Author(s) 2022. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Preparation and characterization of the 3D-printed two-branch nerve conduit. (a) Photograph of the 3D-printed two-branch nerve conduit. (b) Scanning electron microscopy image of the nerve conduit (scale bar = 100 μm). (c) Surface microstructure of the nerve conduit. (d) The live/dead cell imaging kit detects the survival and proliferation of the PC12 cells on GelMA hydrogel at 24 and 48 h. (e) Quantitative proliferation analysis of PC12 cells by CCK-8 assay. Data are presented as the mean ± standard deviation, ^*p < 0.05, ^*^*^*p < 0.001. (f) Compression mechanical properties of the nerve conduit. (g) Representative images of observation (upper) and hematoxylin and eosin staining (lower) of the conduits at 4, 8 and 12 weeks following subcutaneous implantation in rats (star: conduit; scale bar = 50 μm)