Fig. 8.

IL-32 promotes lung metastasis in the EC109 and KYSE150 mouse models in vivo. A EC109 shIL-32 and EC109 shNC (2 × 10⁶ cells/mouse) were intravenously injected into nude mice. Representative images of excised lungs. Graph showed the number of metastatic foci in the lungs (n = 4). B Lung metastasis was confirmed by H&E. Scale bars, 400 or 200 μm. C KYSE150 IL-32β and KYSE150 vector (1 × 10⁶ cells/mouse) were intravenously injected into nude mice. Representative images of excised lungs were shown. D Lung metastasis was confirmed by H&E. Graph showed the number of metastatic foci in the lungs (n = 4). Scale bars, 400 or 200 μm. E, F Immunofluorescence of IL-32 and CD206 in the lungs from mice inoculated with EC109 shIL-32, EC109 shNC, KYSE150 vector and KYSE150 IL-32β cells. Scale bar, 50 μm. G BALB/c nude mice were intravenously injected with 100 µL clodronate liposome or control liposome every 4 days after tail vein injection of 2 × 10⁶ EC109 shNC and EC109 shIL-32 cells until 7 weeks. Representative images of excised lungs. Graph showed the number of metastatic foci in the lungs (n = 5). Data are represented as means ± SEM. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001