Fig. 3.

Effect of fasudil on over-production of ROS induced by LPS in rat PMVECs. A and B LPS induced the production of reactive oxygen species (ROS) in rat PMVECs. Representative fluorescent images of the rat PMVECs and quantitative representation of the relative fluorescence intensity were listed respectively. Values were presented as means ± SD of three independent experiments. ^**P < 0.01 vs the value at LPS 0 h. C and D Fasudil inhibited LPS-induced production of ROS in rat PMVECs. Quiescent PMVECs were incubated with vehicle or fasudil for 2 h, followed by stimulation with 10 μg/ml of LPS for 6 h. Then, intracellular ROS level was detected with DCFH-DA staining, visualized by confocal microscopy. Representative fluorescent images and quantitative representation of the relative fluorescence intensity were shown. Values were presented as means ± SD of three independent experiments. ^##P < 0.01 vs control group; ^**P < 0.01 vs LPS-alone group