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. 2022 Apr 15;15:12. doi: 10.1186/s13072-022-00442-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Analysis of differentially expressed genes between Sus scrofa ICSI- and mICSI-derived blastocysts. A Functional annotations of DEGs in the biological process subcategory cellular process (GO:0009987). B Heatmap displaying mtDNA-encoded genes in Sus scrofa ICSI- and mICSI-derived blastocysts. Rows and columns show individual genes and RNAseq samples, respectively, ordered by position in the Sus scrofa mtDNA sequence. Each tile in the main matrix represents the levels of expression of a single gene in a single RNAseq data set. Colour of tile indicates levels of expression, and a scale is presented on the right. The DNA strand of encoded genes, biotype of genes, and start position in mtDNA are also presented in colour tiles on the left. C and D Analysis of signalling pathways for differentially expressed genes using IPA. Identified signalling network 1 (C) and network 2 (D) are shown. Types of molecules are drawn in different shapes and genes that are up- or down-regulated in mICSI-derived blastocysts are indicated in red and green, respectively