Table 2.
Differentially expressed genes between ICSI- and mICSI-derived blastocysts
| Gene_ida | Chr/Scafoldb | Start | End | Strand | Gene_symbol | Gene_biotype | Description | logFCc | adj.P.Vald |
|---|---|---|---|---|---|---|---|---|---|
| Upregulated in mICSI-derived blastocysts relative to ICSI | |||||||||
| ENSSSCG00000050793 | 9 | 47,00,784 | 47,00,878 | − | ssc-mir-10390 | miRNA | ssc-mir-10390 [Source:miRBase;Acc:MI0033404] | 8.41 | 1.46E−08 |
| ENSSSCG00000030088 | AEMK02000569.1 | 7,21,734 | 7,43,707 | + | protein_coding | colony stimulating factor 2 receptor alpha subunit [Source:NCBI gene;Acc:100620339] | 6.16 | 4.90E−02 | |
| ENSSSCG00000045022 | 14 | 7,38,21,782 | 7,38,28,231 | + | lncRNA | NULL | 6.02 | 1.30E−02 | |
| ENSSSCG00000045442 | AEMK02000694.1 | 25,505 | 52,916 | − | protein_coding | leucine carboxyl methyltransferase 1-like [Source:NCBI gene;Acc:100625764] | 6.00 | 1.30E−02 | |
| ENSSSCG00000021584 | 15 | 12,09,85,245 | 12,09,91,318 | + | CDK5R2 | protein_coding | cyclin dependent kinase 5 regulatory subunit 2 [Source:HGNC Symbol;Acc:HGNC:1776] | 5.67 | 2.94E−02 |
| ENSSSCG00000047552 | 9 | 7,63,60,171 | 7,63,60,887 | + | protein_coding | NULL | 5.40 | 2.94E−02 | |
| ENSSSCG00000016816 | 16 | 1,95,47,009 | 1,99,20,515 | − | ADAMTS12 | protein_coding | ADAM metallopeptidase with thrombospondin type 1 motif 12 [Source:HGNC Symbol;Acc:HGNC:14605] | 5.16 | 2.94E−02 |
| ENSSSCG00000045298 | 9 | 7,73,07,796 | 7,73,15,488 | − | lncRNA | NULL | 4.80 | 4.41E−02 | |
| ENSSSCG00000051325 | 1 | 24,83,28,317 | 24,84,48,089 | − | lncRNA | NULL | 4.31 | 4.96E−02 | |
| ENSSSCG00000010017 | 14 | 4,78,79,317 | 4,79,02,699 | + | SMTN | protein_coding | smoothelin [Source:NCBI gene;Acc:414369] | 3.56 | 1.41E−03 |
| ENSSSCG00000043834 | AEMK02000692.1 | 81,135 | 86,443 | + | protein_coding | NULL | 3.46 | 3.82E−03 | |
| ENSSSCG00000017592 | 12 | 2,74,50,805 | 2,75,26,985 | − | MBTD1 | protein_coding | mbt domain containing 1 [Source:HGNC Symbol;Acc:HGNC:19866] | 2.93 | 3.72E−03 |
| ENSSSCG00000015729 | 15 | 2,96,75,820 | 2,97,14,079 | − | TSN | protein_coding | translin [Source:HGNC Symbol;Acc:HGNC:12379] | 2.68 | 7.98E−04 |
| ENSSSCG00000046732 | AEMK02000589.1 | 1,03,902 | 1,10,514 | + | lncRNA | NULL | 2.65 | 3.45E−02 | |
| ENSSSCG00000050138 | AEMK02000256.1 | 1,71,630 | 1,81,191 | + | lncRNA | NULL | 2.49 | 1.25E−03 | |
| ENSSSCG00000014362 | 2 | 14,21,02,456 | 14,21,15,344 | − | HBEGF | protein_coding | heparin binding EGF-like growth factor [Source:HGNC Symbol;Acc:HGNC:3059] | 2.29 | 1.49E−02 |
| ENSSSCG00000031712 | 5 | 6,27,42,315 | 6,27,59,190 | + | MFAP5 | protein_coding | microfibril associated protein 5 [Source:HGNC Symbol;Acc:HGNC:29673] | 2.06 | 2.94E−02 |
| ENSSSCG00000007043 | 17 | 1,44,20,656 | 1,44,76,189 | − | GPCPD1 | protein_coding | glycerophosphocholine phosphodiesterase 1 [Source:HGNC Symbol;Acc:HGNC:26957] | 1.79 | 4.18E−02 |
| ENSSSCG00000015604 | 9 | 13,15,47,084 | 13,15,60,527 | + | NEK2 | protein_coding | NIMA related kinase 2 [Source:HGNC Symbol;Acc:HGNC:7745] | 1.57 | 4.90E−02 |
| ENSSSCG00000011704 | 13 | 8,97,42,893 | 8,98,89,187 | − | WWTR1 | protein_coding | WW domain containing transcription regulator 1 [Source:HGNC Symbol;Acc:HGNC:24042] | 1.33 | 3.29E−02 |
| ENSSSCG00000015144 | 9 | 5,02,58,971 | 5,05,09,799 | + | GRAMD1B | protein_coding | GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214] | 1.28 | 2.55E−02 |
| ENSSSCG00000020927 | 13 | 10,95,87,256 | 10,96,23,787 | − | SLC2A2 | protein_coding | solute carrier family 2 member 2 [Source:NCBI gene;Acc:397429] | 1.26 | 9.43E−03 |
| ENSSSCG00000034282 | 15 | 11,72,44,763 | 11,74,51,261 | − | ABCA12 | protein_coding | ATP binding cassette subfamily A member 12 [Source:HGNC Symbol;Acc:HGNC:14637] | 1.09 | 4.96E−02 |
| ENSSSCG00000030211 | 12 | 1,97,55,737 | 1,97,88,952 | − | NBR1 | protein_coding | NBR1 autophagy cargo receptor [Source:HGNC Symbol;Acc:HGNC:6746] | 1.07 | 1.67E−02 |
| Downregulated in mICSI-derived blastocysts relative to ICSI | |||||||||
| ENSSSCG00000018062 | MT | 2206 | 2273 | + | Mt_tRNA | product = tRNA-Val | − 11.31 | 4.32E−06 | |
| ENSSSCG00000018070 | MT | 6129 | 6196 | + | Mt_tRNA | product = tRNA-Trp | − 8.93 | 1.34E−04 | |
| ENSSSCG00000018079 | MT | 8891 | 8957 | + | Mt_tRNA | product = tRNA-Lys | − 8.81 | 2.02E−05 | |
| ENSSSCG00000018073 | MT | 6379 | 6444 | − | Mt_tRNA | product = tRNA-Cys | − 8.30 | 1.55E−04 | |
| ENSSSCG00000018071 | MT | 6203 | 6270 | − | Mt_tRNA | product = tRNA-Ala | − 7.55 | 8.92E−04 | |
| ENSSSCG00000032749 | 8 | 8,93,21,491 | 8,93,35,395 | + | PCDH18 | protein_coding | protocadherin 18 [Source:HGNC Symbol;Acc:HGNC:14268] | − 6.48 | 9.43E−03 |
| ENSSSCG00000018072 | MT | 6272 | 6346 | − | Mt_tRNA | product = tRNA-Asn | − 6.10 | 6.78E−03 | |
| ENSSSCG00000012257 | X | 3,89,29,231 | 3,90,06,222 | + | protein_coding | monoamine oxidase A [Source:NCBI gene;Acc:414424] | − 5.94 | 3.09E−02 | |
| ENSSSCG00000051497 | 5 | 10,21,87,453 | 10,27,20,929 | − | lncRNA | NULL | − 5.77 | 7.62E−03 | |
| ENSSSCG00000021343 | 15 | 74,99,026 | 76,32,655 | + | ZEB2 | protein_coding | zinc finger E-box binding homeobox 2 [Source:HGNC Symbol;Acc:HGNC:14881] | − 5.56 | 3.45E−02 |
| ENSSSCG00000050427 | 2 | 12,87,99,599 | 12,92,30,191 | − | lncRNA | NULL | − 5.53 | 2.38E−02 | |
| ENSSSCG00000001097 | 7 | 1,95,87,685 | 1,98,38,093 | − | protein_coding | RHO family interacting cell polarization regulator 2 [Source:NCBI gene;Acc:100154661] | − 5.35 | 2.12E−02 | |
| ENSSSCG00000037634 | 7 | 6,24,71,228 | 6,24,79,223 | + | FOXA1 | protein_coding | forkhead box A1 [Source:HGNC Symbol;Acc:HGNC:5021] | − 5.18 | 3.45E−02 |
| ENSSSCG00000001484 | 7 | 2,64,46,306 | 2,65,22,965 | − | TINAG | protein_coding | tubulointerstitial nephritis antigen [Source:HGNC Symbol;Acc:HGNC:14599] | − 5.14 | 4.87E−02 |
| ENSSSCG00000045069 | 7 | 1,68,85,827 | 1,68,91,770 | − | lncRNA | NULL | − 4.96 | 4.56E−02 | |
| ENSSSCG00000018074 | MT | 6444 | 6509 | − | Mt_tRNA | product = tRNA-Tyr | − 4.73 | 2.94E−02 | |
| ENSSSCG00000035347 | 14 | 10,60,13,495 | 10,67,13,121 | + | CYP2C42 | protein_coding | cytochrome P450 C42 [Source:NCBI gene;Acc:403111] | − 3.95 | 3.31E−03 |
| ENSSSCG00000015780 | 15 | 4,50,83,606 | 4,53,01,158 | + | STOX2 | protein_coding | storkhead box 2 [Source:HGNC Symbol;Acc:HGNC:25450] | − 3.41 | 2.94E−02 |
| ENSSSCG00000023204 | 3 | 4,13,37,071 | 4,13,93,997 | + | AXIN1 | protein_coding | axin 1 [Source:HGNC Symbol;Acc:HGNC:903] | − 3.20 | 1.35E−03 |
| ENSSSCG00000039416 | 15 | 1,56,63,494 | 1,56,67,233 | + | CXCR4 | protein_coding | C-X-C motif chemokine receptor 4 [Source:NCBI gene;Acc:396659] | − 2.54 | 2.55E−02 |
| ENSSSCG00000016925 | 16 | 3,72,99,116 | 3,73,05,116 | − | PLK2 | protein_coding | polo like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19699] | − 2.38 | 4.91E−02 |
| ENSSSCG00000022390 | X | 4,17,26,526 | 4,17,49,726 | + | RGN | protein_coding | regucalcin [Source:HGNC Symbol;Acc:HGNC:9989] | − 1.91 | 9.43E−03 |
| ENSSSCG00000006562 | 4 | 9,57,59,073 | 9,58,64,458 | + | GATAD2B | protein_coding | GATA zinc finger domain containing 2B [Source:HGNC Symbol;Acc:HGNC:30778] | − 1.68 | 9.43E−03 |
| ENSSSCG00000004390 | 1 | 7,50,33,098 | 7,51,69,987 | − | SESN1 | protein_coding | sestrin 1 [Source:HGNC Symbol;Acc:HGNC:21595] | − 1.62 | 1.67E−02 |
| ENSSSCG00000026784 | 2 | 7,61,25,926 | 7,61,46,733 | + | LMNB2 | protein_coding | lamin B2 [Source:HGNC Symbol;Acc:HGNC:6638] | − 1.55 | 1.44E−02 |
| ENSSSCG00000014267 | 2 | 13,37,08,266 | 13,39,51,438 | − | protein_coding | Rap guanine nucleotide exchange factor 6 [Source:NCBI gene;Acc:100521255] | − 1.54 | 1.44E−02 | |
| ENSSSCG00000047299 | AEMK02000489.1 | 43,232 | 45,101 | + | RN18S | rRNA | 18S ribosomal RNA [Source:NCBI gene;Acc:100861538] | − 0.71 | 3.89E−06 |
| ENSSSCG00000012847 | 2 | 4,35,125 | 4,73,744 | + | TALDO1 | protein_coding | transaldolase 1 [Source:NCBI gene;Acc:100514210] | 0.85 | 4.90E−02 |
aEnsembl (https://m.ensembl.org/index.html) gene ID
bChromosome or scaffold number where gene is located
cLog 2-fold change relative to ICSI-derived blastocyst
dAdjusted p-value