Figure 2.
NPPB increased tonic currents of dentate gyrus granule cells in acute hippocampal brain slices. A: Tonic currents under control conditions, during NPPB (100 μM) application, and after wash out of NPPB. All points histograms and Gaussian fits used to determine tonic current amplitudes [i.e. change in holding current produced by bicuculline (Bic)] are shown to right of each trace. Tonic current amplitudes are indicated by vertical lines. B: Time course for experiment in panel A. C: Tonic currents in presence of exogenous GABA (10 μM) before and during NPPB application. D: Mean tonic current amplitude under control conditions, in the presence of NPPB, exogenous GABA (GABA), and GABA + NPPB (n = 5 cells for NPPB alone, n = 3 cells with exogenous GABA). ** - p<0.01.