KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-CHCHD10 (1:500) | ProteinTech | Cat# 25671-AP; RRID: AB_2880187 |
| Rabbit anti-CHCHD2 (1:500) | ProteinTech | Cat# 19424-AP; RRID: AB_10638907 |
| Mouse anti-Tim23 (1:1000) | BD Transduction Lab | Cat# 611222; RRID: AB_398754 |
| Mouse anti-VDAC1 (1:1000) | NeuroMabs | Cat# 73–204; RRID: AB_10673517 |
| Rabbit anti-Cyclophilin D (1:000) | Calbiochem | Cat# 239784; RRID: AB_2169445 |
| Mouse anti-GSH (1:500) | Virogen | Cat# 101A; RRID: N/A |
| Rabbit anti-CLPX (1:1000) | Abcam | Cat# ab168338; RRID: N/A |
| Rabbit anti-LONP1 (1:1000) | Abcam | Cat# ab103809; RRID: AB_10858161 |
| Rabbit anti-PMPCB (1:1000) | ProteinTech | Cat# 16064–1-AP; RRID: AB_2167122 |
| Rabbit anti-LC3B (1:1000) | Sigma | Cat# L7543; RRID: AB_796155 |
| Rabbit anti-STOML2 (1:500) | ProteinTech | Cat# 10348–1-AP; RRID: AB_2286822 |
| Rabbit anti-PHB2 (1:500) | Cell Signaling | Cat# 14085; RRID: AB_2798387 |
| Rabbit anti-PRDX4 (1:1000) | Sigma | Cat# SAB4301759; RRID: N/A |
| Rabbit anti-COX6A1 (1:500) | ProteinTech | Cat# 11460–1-AP; RRID: AB_2085445 |
| Rabbit anti-SLC25A20 (CACT) (1:1000) | ProteinTech | Cat# 19363–1-AP; RRID: AB_10642001 |
| Rabbit anti-MAT2B (1:500) | ProteinTech | Cat# 15952–1-AP; RRID: AB_10637268 |
| Rabbit anti-MTR (1:500) | ProteinTech | Cat# 25896–1-AP; RRID: AB_2880287 |
| Rabbit anti-GMPR1/2 (1:500) | ProteinTech | Cat# 15683–1-AP; RRID: AB_2111072 |
| Rabbit anit-ADK (1:5000) | ProteinTech | Cat# 66929–1-Ig; RRID: AB_2882255 |
| Rabbit anti-GMPS (1:500) | ProteinTech | Cat# 16376–1-AP; RRID: AB_2232412 |
| Rabbit anti-Peroirdoxin 2 (PRDX2) (1:1000) | ProteinTech | Cat# 10545–2-AP; RRID: AB_2168202 |
| Goat anti-HSP60 (1:1000) | Enzo Life Sciences | Cat# ADI-SPA828; RRID: AB_10615201 |
| Sheep anti-NOGO-B (RTN4) (1:500) | R&D Systems | Cat# AF6034; RRID: AB_10573837 |
| HRP Donkey anti-Goat (1:10000) | Jackson ImmunoResearch | Cat# 705–035-003; RRID: AB_2340390 |
| HRP Goat anti-Mouse (1:5000–10000) | Jackson ImmunoResearch | Cat# 115–035-146; RRID: AB_2307392 |
| HRP Goat anti-Rabbit (1:5000–10000) | Jackson ImmunoResearch | Cat# 111–035-144; RRID: AB_2307391 |
| HRP Donkey anti-Sheep (1:5000) | Jackson ImmunoResearch | Cat# 713–035-003; RRID: AB_2340709 |
|
Chemicals, peptides, and recombinant proteins | ||
| TRIzol | Life Technology | Cat# 15596–026 |
| Methanol | VWR | Cat# BDH1135–4LP |
| Trypsin-EDTA | Invitrogen | Cat# 25200–072 |
| PBS (10X), pH 7.4 | ThermoFisher Scientific | Cat# 70011044 |
| Malate | Sigma | Cat# 46940-U |
| Pyruvate | Sigma | Cat# P2256 |
| Succinate | Sigma | Cat# W327700 |
| Glutamate | Sigma | Cat# 49621 |
| D-mannitol, BioUltra | Sigma | Cat# M4125 |
| Sucrose, BioUltra | Sigma | Cat# 84097 |
| Hepes | Sigma | Cat# H3375 |
| EGTA | Sigma | Cat# E4378 |
| EDTA | Sigma | Cat# EDS |
| SF-6847 | Selleckchem | Cat# S2895 |
| Carboxyatractyloside potassium salt | Sigma | Cat# C4992 |
| BSA, essentially fatty acid free | Sigma | Cat# A6003 |
| BSA | Sigma | Cat# A7906 |
| Amplex UltraRed Reagent | ThermoFisher Scientific | Cat# A36006 |
| Horseradish peroxidase | Sigma | Cat# P6782 |
| Tween-20 | Sigma | Cat# P7949 |
| NP-40 | BioChemika | Cat# 74385 |
| Maxima SYBR Green/ROX qPCR Master Mix (2X) | ThermoFisher Scientific | Cat# K0222 |
| ATP | Sigma | Cat# 8937 |
| ADP | Sigma | Cat# A5285 |
| Diadenosine pentaphosphate | Sigma | Cat# D4022 |
| MgCl2 | ThermoFisher Scientific | Cat# AM9530G |
| KCl | Sigma | Cat# 58221 |
| Tris-HCl | Sigma | Cat# 10812846001 |
| Rotenone | Sigma | Cat# R8875 |
| Hydrogen peroxide | Sigma | Cat# H1009 |
| 1-propanol | Sigma | Cat# 09158 |
| 2-propanol | Sigma | Cat# I9516 |
| Ethanol | Sigma | Cat# E7023 |
| Acetic acid | Sigma | Cat# A6283 |
| Sodium acetate Anhydrous | ThermoFisher Scientific | Cat# S210–500 |
| Coenzyme Q9 | Frontier Specialty Chemicals | Cat# JK158165 |
| Oxalic acid | Sigma | Cat# 75688 |
| Nitric acid | VWR | Cat# 87003–261 |
| Digitonin | Sigma | Cat# D141 |
| NaCl | Sigma | Cat# S9888 |
| Imidazole HCl | Sigma | Cat# L143073 |
| Bis-Tris | Sigma | Cat# B9754 |
| 6-aminohexanoic acid | Sigma | Cat# 07260 |
| SimplyBlue SafeStain | ThermoFisher Scientific | Cat# LC6060 |
| Carbonyl iron | Sigma | Cat# C3518 |
| 4X Laemmli Sample Buffer | Bio-Rad | Cat# 1610747 |
| 2-Mercaptoethanol | Sigma | Cat# M6250 |
|
Critical commercial assays | ||
| Protein Carbonyl Assay Kit (Western Blot) | Abcam | Cat# ab178020 |
| Lipid Peroxidation (MDA) Assay Kit | Abcam | Cat# ab118970 |
| RNeasy Micro Kit | Qiagen | Cat# 74104 |
| ImProm-II Reverse Transcriptase System | Promega | Cat# A3800 |
| Quick Start Bradford Protein Assay | Bio-Rad | Cat# 500–0201 |
| Wizard Genomic DNA Purification Kit | Promega | Cat# A1120 |
| Bradford Protein Assay | Bio-Rad | Cat# 5000001 |
|
Deposited data | ||
| Mouse heart RNA-seq data | Gene Expression Omnibus gene repository | GEO: GSE193801 |
| Mouse heart metabolomics data | Metabolomics Workbench | Study ID: ST002068 |
| Mouse heart proteomics data | ProteomeXchange | Identifier: PDX031444 |
|
Experimental models: Organisms/strains | ||
| C57BL/6NJ-Chchd10em8Lutzy/J | The Jackson Laboratory | Stock No. 028952 |
|
Software and algorithms | ||
| Prism v9 | GraphPad Software | N/A |
| Fiji/ImageJ software | NIH | N/A |
| Image Studio | LI-COR Biosciences | N/A |
| Image Lab | Bio-Rad | N/A |
| R | The R Foundation | N/A |
| STAR aligner v2.5.0a | GenSoft | N/A |
| HTSeq-count v0.6.1 | Simon Anders | N/A |
| DESeq2 v1.30.0 | Bioconductor | N/A |
| MetaboAnalyst v5.0 | Jianguo Xia | N/A |