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. Author manuscript; available in PMC: 2022 Apr 16.
Published in final edited form as: Mol Cancer Ther. 2021 Jul 12;20(10):1800–1808. doi: 10.1158/1535-7163.MCT-21-0174

Figure 1.

Figure 1.

Inhibition of WNK1 decreases breast cancer cell migration. A, Wound closure of MCF-DCIS cells transfected with control or WNK1 siRNA and grown to confluence. Representative images 24 hours after wounding are shown. Graph shows quantification of relative wound area (mean ± SD, n = 6 wounds). B, Wound closure of MCF-DCIS cells grown to confluence and treated with DMSO or SW133708 (10 μmol/L) or cytochalasin D immediately after wounding. Representative images 24 hours after wounding are shown. Graph shows quantitation of wound area (mean ± SD, n = 6 wounds). C, BT-549, BT-20, HCC1569, and HCC1419 cells were grown to confluence, treated with DMSO, WNK463 (1 μmol/L), or cytochalasin D (1 μmol/L) and imaged at time 0 and over 6 time points ending at 48 hours. Results are from n = 8 and are shown as mean ± SE. The last two time points from each treatment were used to determine statistical significance analyzed by Student t test (*, P < 0.05; ***, P < 0.0001).