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. 2022 Jan 7;25(3):515–526. doi: 10.1007/s10120-021-01275-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Co-culture of YTN16 cells with LmcMF cells promotes the migration of M2 macrophage. Transwell migration assay showed that the addition of YTN16 cell-conditioned medium (CM) to LmcMF cells enhanced M2 macrophage migration, while Tranilast administration (50 μM) significantly inhibited the migration of M2 macrophages stimulated by LmcMF cells and YTN16 cells-CM. Migrating cells were counted in four randomly chosen fields. Data represent the mean ± SEM of triplicate wells from three independent experiments (**p < 0.01)